GPVI Interaction with Polymerized Fibrin Boosts Thrombin Generation and Thrombus Growth
نویسندگان
چکیده
منابع مشابه
Platelet glycoprotein VI binds to polymerized fibrin and promotes thrombin generation.
Fibrin, the coagulation end product, consolidates the platelet plug at sites of vascular injury and supports the recruitment of circulating platelets. In addition to integrin αIIbβ3, another as-yet-unidentified receptor is thought to mediate platelet interaction with fibrin. Platelet glycoprotein VI (GPVI) interacts with collagen and several other adhesive macromolecules. We evaluated the hypot...
متن کاملThrombin binding to GPIbα induces platelet aggregation and fibrin clot retraction supported by resting αIIbβ3 interaction with polymerized fibrin
Thrombin plays a pivotal role in haemostasis and thrombosis as the main effector protease of the coagulation cascade converting circulating fibrinogen into fibrin. Thrombin is also a powerful activator of platelets, inducing platelet shape change, release of the granular content, integrin αIIbβ3 activation and platelet aggregation (1). Since thrombin and platelets are critical in the developmen...
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Recent data indicate an important contribution of coagulation factor (F)XII to in vivo thrombus formation. Because fibrin structure plays a key role in clot stability and thrombosis, we hypothesized that FXII(a) interacts with fibrin(ogen) and thereby regulates clot structure and function. In plasma and purified system, we observed a dose-dependent increase in fibrin fiber density and decrease ...
متن کاملThrombus growth and embolism on tissue factor-bearing collagen surfaces under flow: role of thrombin with and without fibrin.
OBJECTIVE At sites of vascular injury, thrombin is an important mediator in thrombus growth and stability. Using microfluidic flow devices as well as patterned surfaces of collagen and tissue factor (TF), we sought to determine the role that fibrin plays in clot stability without interfering with the production of thrombin. METHODS AND RESULTS We deployed an 8-channel microfluidic device to s...
متن کاملFibrin and D-dimer bind to monomeric GPVI.
Fibrin has recently been shown to activate platelets through the immunoglobulin receptor glycoprotein VI (GPVI). In the present study, we show that spreading of human platelets on fibrin is abolished in patients deficient in GPVI, confirming that fibrin activates human platelets through the immunoglobulin receptor. Using a series of proteolytic fragments, we show that D-dimer, but not the E fra...
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ژورنال
عنوان ژورنال: Blood
سال: 2014
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v124.21.4152.4152